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Evaluation of Fosfomycin Activity Against Extended Spectrum Beta Lactamase (ESBL) Producing Enterobacteriaceae Isolated from Three Centers of Tehran, Iran

[ Vol. 13 , Issue. 2 ]

Author(s):

Roya Ghanavati, Elnaz O. Digesara, Hossein Kazemian, Fateme Yazdani, Alireza Torki, Behrooz S. Kalani and Gholamreza Irajian*   Pages 180 - 186 ( 7 )

Abstract:


Background: Rising rates of antimicrobial resistance among Enterobacteriaceae limit the use of reliably active forms of available drugs. The aim of this study was to inves-tigate the prevalence of fosfomycin (US6794490B2) resistance gene among ESBL produc-ing isolates in Iran.

Method: We tested 355 isolates of Enterobacteriacea collected from various clinical sam-ples including urine, wounds, blood and other sources during June 2016 to July 2017. Anti-biotic sensitivity and extended spectrum beta lactamase (ESBL) production were tested us-ing agar dilution method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBL genes (blaTEM, bla SHV,bla CTX-M), plasmid-encoded fosfomycin re-sistance genes (fosA, fosB, fosA3 and fosC2) and chromosomal mutations (murA, glpT, uhpT) were detected by polymerase chain reaction (PCR).

Results: In this study, 151 of the 355 isolates were ESBL-positive. blaCTX-M (77%) was the most common gene followed by blaSHV (70%) and blaTEM (58%), either alone or in combina-tion. Eighty nine percent (132/151) of the ESBL-positive isolates were MDR. Antimicrobial susceptibility rates were higher for fosfomycin (92.8%) and imipenem (35.5%) among ESBL-positive isolates. None of the ESBL- positive isolates harbored any mutations or plasmid-mediated fosfomycin resistance determinants.

Conclusion: In conclusion, fosfomycin showed good antimicrobial activity against multi-drug resistance ESBL- positive Enterobacteriaceae.

Keywords:

Fosfomycin, ESBL, ESBL- positive Enterobacteriaceae, fos gene, phosphonic acid, infections.

Affiliation:

Department of Microbiology, School of Medicine, Iran University of Medical Sciences ,Tehran, Department of Microbiology, School of Medicine, Iran University of Medical Sciences ,Tehran, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Department of Microbiology, School of Medicine, Iran University of Medical Sciences ,Tehran, Department of Microbiology, School of Medicine, Iran University of Medical Sciences ,Tehran, Department of Microbiology, School of Medicine, Iran University of Medical Sciences ,Tehran



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