Jalil Kardan-Yamchi, Mohaddese Mahboubi, Hossein Kazemian, Gholamreza Hamzelou and Mohammad M. Feizabadi* Pages 68 - 74 ( 7 )
Background: Microbial resistance to antibiotics and their adverse effects related to these antibiotics are a matter of global public health in the 21th century. The emergence of drug-resistant strains, has gained the interest of the scientists to discover new antimicrobial agents from the essential oil of medicinal plants.Methods: Anti-mycobacterial effects of Trachyspermum copticum and Pelargonium graveolens essential oils were determined against multi-drug resistant clinical strains of Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium fortuitum and standard strain of Mycobacterium tuberculosis H37Rv by a Broth micro-dilution method. Pelargonium graveolens plant named Narmada was discovered by Kulkarni R.N et al. (Patent ID, USPP12425P2) and a formulation comprising thymol obtained from Trachyspermum is useful in the treatment of drug-resistant bacterial infections (Patent ID, US6824795B2). The chemical composition of hydro-distilled essential oils was determined by GC and GC-MS. Results: Minimum Inhibitory Concentration (MIC) values for T. copticum essential oil against tested isolates were ranged from 19.5 µg/mL to 78 µg/mL. The least minimum inhibitory concentration of P. graveolens extract against M. Kansasii and MDR-TB was 78 µg/ml. Conclusion: The results of the present research introduced T. copticum and P. graveolens essential oils as a remarkable natural anti-mycobacterial agent, but more pharmacological studies are required to evaluate their efficacy in animal models.
Trachyspermum copticum, Pelargonium graveolens, essential oil, antimycobacterial activity, Mycobacterium tuberculosis, chemical composition.
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Medicinal Plants Research Department, Research and Development, TabibDaru Pharmaceutical Company, Kashan, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Tehran Regional Reference Laboratory for Tuberculosis, Tehran, Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran